2011 Established Investigator
TITLE: Professor Emeritus of Laboratory Medicine and Pathobiology, Senior Scientist
INSTITUTION: The University of Toronto
STUDY TITLE: Hydrocephalus and lymphatic cerebrospinal fluid absorption
Miles Johnston, BSc, PhD, received a BS in physiology/pharmacology and a PhD in the Division of Experimental Pathology and Department of Pathology at the University of Toronto. He subsequently completed his Doctoral Training at the A.R.C Institute of Animal Physiology in Cambridge, England. Dr. Johnston has been the Senior Scientist of Clinical Integrative Biology at the Sunnybrook Research Institute and Professor of Laboratory Medicine and Pathobiology at the University of Toronto. Dr. Johnston received an Established Investigator award in 2011 for his study, Hydrocephalus and lymphatic cerebrospinal fluid absorption.
His research investigated CSF transport and testing the hypothesis that discontinuity between CSF compartment and extracranial lymphatic vessels leads to hydrocephalus. The major objective of this research is to test if the lymphatic vessels responsible for CSF absorption respond to pharmacological agents. If the results are in the affirmative, this raises the intriguing possibility that we may be able to modulate lymphatic pumping activity to facilitate CSF removal from the subarachnoid space and in doing so, stop or reverse ventricular expansion in hydrocephalus with pharmaceutical approaches.
Selected hydrocpehalus-related articles
Baker A, Semple JL, Moore S, Johnston M.
Lymphatic function is impaired following irradiation of a single lymph node.
Lymphat Res Biol. 2014 Jun;12(2):76-88.
Kim H, Moore SA, Johnston MG.
Potential for intranasal drug delivery to alter cerebrospinal fluid outflow via the nasal turbinate lymphatics.
Fluids Barriers CNS. 2014 Feb 15;11(1):4
Johnston MG, Del Bigio MR, Drake JM, Armstrong D, Di Curzio DL, Bertrand J.
Pre- and post-shunting observations in adult sheep with kaolin-induced hydrocephalus.
Fluids Barriers CNS. 2013 Jul 11;10:24
Nagra, G., Li, J., McAllister, P., Wagshul, M., Johnston, M.
Elevated CSF outflow resistance associated with impaired lymphatic CSF absorption in a rat model of kaolin-induced communicating hydrocephalus.
Cerebrospinal Fluid Research, 7:4, 2010
Amy Baker, Harold Kim, John L Semple, Dan Dumont, Molly Shoichet, Dalia Tobbia, Miles Johnston.
Experimental assessment of pro-lymphangiogenic growth factors in the treatment of post-surgical lymphedema following lymphadenectomy.
Breast Cancer Res. 2010; 12(5): R70
Tobbia, D., Semple, J., Baker, A., Semple, A., Dumont, D., Johnston, M.
Lymphatic Function and Lymphedema Development in Sheep Following Lymph Node Excision.
Journal of Vascular Research, 46: 426-434, 2009.
Tobbia, D., Semple, J., Baker, A., Dumont, D., Johnston, M.
Experimental assessment of autologous lymph node transplantation as treatment of post-surgical lymphedema.
Plastic and Reconstructive Surgery, 124: 777-786, 2009.
Yücel, Y., Johnston, M., Ly, T., Patel, M., Drake, B., Gümüş, E., Fraenkl, S.A., Moore, S., Tobbia, D., Armstrong, D.,Horvath, E., Gupta, N.
Identification of lymphatics in the ciliary body of the human eye: Uveolymphatic Outflow Pathway.
Experimental Eye Research, 89: 810-819, 2009. (Epub 2009 Sept 1)
Nagra, G., Koh, L., Kim, M., Aubert, I., Johnston, M.
Intraventricular injection of antibodies to β1 integrins generates pressure gradients in the brain favoring hydrocephalus development in rats.
American Journal of Physiology, 297, R1312-R1321, 2009
Li, J., Shen, Y., McAllister II, J.P., Wagshul, M.E., Miller, J.M., Egnor, M., Johnston, M.G., Haacke, M., Walker, M.L.
Communicating hydrocephalus in adult rats with obstruction of the basal cisterns or the cortical subarachnoid space.
Experimental Neurology, 211: 351-361, 2008.
Nagra, G., Li, J., McAllister, P., Miller, J., Wagshul, ME., Johnston, M.
Impaired lymphatic cerebrospinal fluid absorption in a rat model of kaolin-induced communicating hydrocephalus.
American Journal of Physiology, 294: R1752-R1759, 2008.